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Purification of Plasmid DNA by CsCl/Ethidium Bromide

Insoluble in water; Very soluble in acetonitrile and methanol; Almost completely(>99.5) in ethanol. mg/mL (20 °C). Tetracycline antibiotics (tRNA binding).

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Immobilized metal ion affinity chromatography (IMAC)

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The results of our experiments show that at high tetracycline hydrochloride. The antibiotic treatment at both 5 and 1 mg/ml resulted in exclusively male progeny.

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